[Ultramicromethod of fractionating acid proteins on polyacrylamide gels]

A simple method is suggested for fractionation of acid proteins. The method sensitivity is 10(-11) g per single band. Since the available modifications of capillary microelectrophoresis are very complicated to apply to an analysis of protein picograms, such a method was badly needed. Fractionation of the samples is made on polyacrylamide gel tubes up to 40 microns in diameter. An original buffer system is employed. The possibility of fractionating acid proteins from vertebrate animal brain has been demonstrated.