Developmental changes in the pattern of amino acid transport at the blood-brain barrier in rats

Oldendorf's method has been widely used to estimate and characterize the transport of amino acids across the blood-brain barrier in rats. However, it cannot be used with very young animals. A modification of this method (retrograde injection into the right brachial artery, instead of orthograde injection into the common carotid artery) allowed the estimation of the brain uptake index of some amino acids in 5-, 12- and 19-day-old rats, as well as the study of self- and cross-inhibition and of sodium dependency. The results obtained showed that the pattern of transport of amino acids was different in 5-day-old and in 19-day-old rats. In young rats, besides the presence of the L-system, which transported large neutral amino acids as in adult rats, the presence of another system of transport for neutral amino acids was strongly suggested. The activity of this system which transported alanine, serine, cysteine and threonine, decreased during development and it had many of the characteristics of the ASC system described by Christensen. In addition, the presence of a system of transport for beta-amino acids at the blood-brain barrier is suggested.