Conjugate internalization of apposed plasma membranes in mouse olfactory bulb during postnatal development
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Abstract:
Apposed plasma membranes of mitral cells and granule cell gemmules of mouse olfactory bulb are internalized in a conjugate fashion into mitral cell perikarya during postnatal development. Such conjugate internalization of plasma membranes occurs by way of double-walled coated vesicles (DWCVs) and becomes accelerated between 16 and 37 days of postnatal age. The formation of DWCVs appears to be a mechanism for the internalization of intercellularly adhered plasma membranes.
Insights
During mouse olfactory bulb development, mitral cells internalize adjacent granule cell membranes via double-walled coated vesicles. This conjugate membrane internalization process accelerates significantly in early postnatal life.
Area of Science:
- Neuroscience
- Cell Biology
- Developmental Biology
Background:
- The olfactory bulb is crucial for processing scent information.
- Neuronal development involves complex cellular interactions and membrane dynamics.
- Understanding membrane trafficking is key to comprehending neuronal maturation.
Purpose of the Study:
- To investigate the mechanism of plasma membrane internalization between mitral cells and granule cell gemmules.
- To characterize the role of double-walled coated vesicles (DWCVs) in this process.
- To determine the developmental timing of conjugate membrane internalization.
Main Methods:
- Microscopic analysis of mouse olfactory bulb tissue during postnatal development.
- Observation of plasma membrane apposition and internalization events.
- Identification and characterization of double-walled coated vesicles (DWCVs).
Main Results:
- Apposed plasma membranes of mitral cells and granule cell gemmules are internalized together into mitral cell perikarya.
- This conjugate internalization occurs via the formation of double-walled coated vesicles (DWCVs).
- The rate of DWCV formation and membrane internalization accelerates between 16 and 37 days of postnatal age.
Conclusions:
- Double-walled coated vesicles (DWCVs) represent a novel mechanism for the conjugate internalization of adhered intercellular plasma membranes.
- This process is a significant feature of postnatal olfactory bulb development in mice.
- DWCVs play a critical role in remodeling neuronal connections during development.