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Liquid-chromatographic quantification of piracetam
Clinical Chemistry
|April 1, 1983
Summary
This study presents a new method for quantifying piracetam in biological samples using reversed-phase chromatography. The developed technique offers a rapid, inexpensive, and convenient way for clinical laboratories to measure piracetam levels.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Piracetam, a gamma-aminobutyric acid analog, exhibits maximal UV absorption at 197 nm.
- Quantification of piracetam at 197 nm is challenging due to interference from other compounds absorbing in the 190-220 nm range.
Purpose of the Study:
- To develop a reliable method for piracetam detection and quantification in biological extracts.
- To overcome the limitations of direct UV spectrophotometry for piracetam analysis.
Main Methods:
- Methanol extracts of serum and aqueous humor were analyzed using reversed-phase C-18 column chromatography.
- Isocratic elution with KH2PO4 (0.1 mol/L, pH 4.8) was employed.
- Detection and quantification were performed using UV spectrophotometry.
Main Results:
- The chromatographic method allowed for the detection and quantification of piracetam down to 0.2 mmol/L.
- Piracetam exhibited a retention time of approximately 5 minutes under the specified conditions.
- Linear detector response was observed for quantities ranging from 5 to 15 nmol.
Conclusions:
- The developed reversed-phase high-performance liquid chromatography (RP-HPLC) method is suitable for the accurate determination of piracetam in biological samples.
- This method is rapid, cost-effective, and practical for clinical laboratory settings.
- The technique effectively resolves piracetam from interfering substances, enabling precise quantification.