G-bands without pretreatment of slides, in chemically defined conditions

We investigated the capability of Giemsa dyes to produce G-bands without pretreatment of slides in chemically defined conditions. G-banding was produced between pH 7.0 and pH 11.0. Within this range, we observed that the achievement of G-bands was dependent on the ionic strength and time of staining. From these data and from those of other authors on other chemical variables and on the mechanisms of staining, we propose a working hypothesis of G-band formation, with a step involving shrinkage of the chromatids and rearrangement of the chromosome fiber during the staining process.