Ro 22-5417, a new clavam antibiotic from Streptomyces clavuligerus. II. Fermentation, isolation and structure

The purification of a new antibiotic, Ro 22-5417, was achieved by a variety of preparative column chromatographic methods. The antibiotic was obtained crystalline with an overall recovery of 3% after a 300-fold purification. The structure was determined from proton and 13C NMR spectra to be 3-(7-oxo-1-aza-4-oxabicyclo[3.2.0]hept-3-yl)alanine.