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Related Experiment Videos

Fibronectin expression during myogenesis.

J M Gardner, D M Fambrough

    The Journal of Cell Biology
    |February 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Chick muscle cells synthesize fibronectin consistently during differentiation. Myotubes show a longer delay in fibronectin secretion compared to fibroblasts, indicating cell-specific protein processing.

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    Area of Science:

    • Cell Biology
    • Developmental Biology
    • Biochemistry

    Background:

    • Fibronectin is a crucial extracellular matrix protein involved in cell adhesion and migration.
    • Understanding fibronectin's role in muscle differentiation is key to comprehending tissue development and repair.

    Purpose of the Study:

    • To investigate the biosynthesis and localization of fibronectin during chick muscle cell differentiation.
    • To precisely analyze fibronectin expression in myoblasts and myotubes using specific antibodies.

    Main Methods:

    • Utilized two monoclonal antibodies: one for selective cell killing and another specific for avian fibronectin.
    • Established well-defined cultures of myoblasts and myotubes to avoid contamination.
    • Quantified fibronectin synthesis as a fraction of total protein synthesis.

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  • Measured the lag period between fibronectin biosynthesis and secretion.

    • Main Results:

    • Fibronectin synthesis remained constant (0.3-0.4% of total protein) before and after myoblast fusion.
    • Absolute fibronectin synthesis may increase in myotubes as they accumulate myofibrillar proteins.
    • A 2-3 hour lag period for fibronectin secretion was observed in myotubes, distinct from the 30-minute lag in fibroblasts.
    • This intracellular transit time is cell-type specific, not protein-specific.

    Conclusions:

    • Fibronectin biosynthesis rate is maintained during muscle differentiation.
    • Myogenic cells exhibit a characteristic intracellular transit time for secretory proteins, including fibronectin.
    • The findings provide insights into the regulation of extracellular matrix production during muscle development.