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Lymphocyte migration into three-dimensional collagen matrices: a quantitative study.

S L Schor, T D Allen, B Winn

    The Journal of Cell Biology
    |April 1, 1983
    PubMed
    Summary
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    Lymphocytes migrate through collagen gels via a random-walk mechanism, without needing serum or collagenase. This provides a model for studying cell-matrix interactions in lymphocyte recirculation.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biophysics

    Background:

    • Lymphocyte recirculation is crucial for immune surveillance.
    • Understanding cell-matrix interactions is key to deciphering lymphocyte behavior in vivo.
    • Existing models may not fully capture the complexities of lymphocyte migration within native tissue environments.

    Purpose of the Study:

    • To develop a quantitative model system for studying lymphocyte interactions within a three-dimensional collagen matrix.
    • To investigate the mechanisms of lymphocyte penetration and distribution in native collagen fibers.
    • To analyze the kinetics of lymphocyte migration to understand cell-matrix and cell-cell interactions relevant to in vivo recirculation.

    Main Methods:

    • Quantitative analysis of lymphocyte distribution within three-dimensional native collagen gels over time.

    Related Experiment Videos

  • Assessment of lymphocyte penetration kinetics.
  • Microscopic observation of cell behavior within the collagen matrix.

    • Main Results:

    • Lymphocytes can be quantitatively plated and their distribution tracked within 3D collagen gels.
    • Lymphocyte penetration into the collagen matrix does not require collagenolytic activity or serum.
    • Kinetics of penetration suggest lymphocytes migrate in a random-walk fashion.

    Conclusions:

    • The developed model system allows for quantitative study of lymphocyte behavior in a 3D collagen environment.
    • Lymphocyte migration in this model is independent of collagenase activity and serum, suggesting intrinsic migratory properties.
    • The random-walk migration pattern provides insights into cell-matrix interactions influencing lymphocyte recirculation in vivo.