Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method for determining thiopental in plasma.

J R Sharman, K M Ahern

    Journal of Analytical Toxicology
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cyanide poisoning of cattle grazing "reed sweet-grass"

    New Zealand veterinary journal·2011
    Same author

    Serum aminoglycoside clearance is predicted as poorly by renal aminoglycoside clearance as by creatinine clearance in critically ill patients.

    Critical care medicine·1991
    Same author

    Variability in the elimination of mianserin in elderly patients.

    British journal of clinical pharmacology·1989
    Same author

    Circadian pharmacokinetics of methotrexate.

    Cancer chemotherapy and pharmacology·1989
    Same author

    An enzymatic method for erythrocyte acetylcholinesterase.

    Clinical chemistry·1988
    Same author

    Co-administered oral metoclopramide does not enhance the rate of absorption of oral diazepam.

    Anaesthesia and intensive care·1988

    A new, rapid High-Performance Liquid Chromatography (HPLC) method accurately measures thiopental in plasma. This technique is suitable for monitoring patients receiving thiopental sedation and can be enhanced for sensitive studies.

    Area of Science:

    • Analytical Chemistry
    • Pharmacology

    Background:

    • Thiopental is a widely used anesthetic agent.
    • Accurate quantification of thiopental in biological samples is crucial for therapeutic drug monitoring and pharmacokinetic studies.

    Purpose of the Study:

    • To develop and validate a simple, rapid High-Performance Liquid Chromatography (HPLC) method for measuring thiopental concentrations in human plasma.
    • To establish the suitability of the method for clinical applications, including monitoring patients under thiopental infusion.

    Main Methods:

    • Plasma samples were deproteinized using acetonitrile and methanol, with flufenamic acid as an internal standard.
    • Chromatographic separation was performed on a C-18 reverse-phase column.
    • Detection was achieved at 280 nm, leveraging the absorption maxima of both thiopental and flufenamic acid.

    Related Experiment Videos

    Main Results:

    • The developed HPLC method demonstrated linearity up to 75 micrograms/mL.
    • The method is rapid and simple, making it practical for routine laboratory use.
    • Detection sensitivity can be further improved for specialized pharmacokinetic investigations.

    Conclusions:

    • This validated HPLC method provides a reliable and efficient means for quantifying thiopental in plasma.
    • The method is well-suited for therapeutic drug monitoring in patients sedated with thiopental.
    • Potential for enhanced sensitivity allows for detailed pharmacokinetic analyses, including single-dose studies.