Stereospecific, reversible binding of D-[3H]glucose to crude membrane fractions prepared from rat brain

To a crude preparation of synaptic membranes prepared from rat brain, stereospecific, saturable, reversible binding was described of D-[3H]glucose. Binding showed a Kd = 0.45 microM and the fractional rate of dissociation was approximately eight times the fractional rate of association. D-[3H]glucose binding was displaced by 2-deoxyglucose and 3-O-methylglucose and it was abolished when membranes were denatured by heating.