Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Unsaturated fatty acids in the postnatally developing rat lung.

J P Kehrer, A P Autor

    Lipids
    |January 1, 1983
    PubMed
    Summary

    Neonatal rat lungs prioritize polyunsaturated fatty acids (PUFA) for phospholipid synthesis. Adult lungs esterify PUFA to glycerol, indicating developmental changes in fatty acid metabolism.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Differential toxicities of cyclophosphamide and its glutathione metabolites to A549 cells.

    Toxicology in vitro : an international journal published in association with BIBRA·2010
    Same author

    Roles of 5-lipoxygenase activating protein in cell proliferation and apoptosis.

    Cell biology and toxicology·2003
    Same author

    Report by the editors.

    Toxicology letters·2003
    Same author

    Acute exposure of cerebellar granule neurons to ethanol suppresses stress-activated protein kinase-1 and concomitantly induces AP-1.

    Toxicology and applied pharmacology·2001
    Same author

    Proteolytic loss of bcl-x(L) in FL5.12 Cells undergoing apoptosis induced by MK886.

    Toxicology and applied pharmacology·2001
    Same author

    Inhibition of peroxisome-proliferator-activated receptor (PPAR)alpha by MK886.

    The Biochemical journal·2001

    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Pulmonology

    Background:

    • Fatty acid desaturase activity is crucial for synthesizing polyunsaturated fatty acids (PUFA).
    • Lung tissue composition and function undergo significant changes from neonate to adult stages.
    • Dietary changes, such as weaning, impact fatty acid profiles in developing mammals.

    Purpose of the Study:

    • To investigate the developmental changes in C-9 fatty acid desaturase activity in rat lungs.
    • To determine the incorporation patterns of specific PUFA (linoleate, linolenate, arachidonate) into lipids in neonatal versus adult rat lungs.
    • To elucidate the differential roles of PUFA in lung lipid metabolism during development.

    Main Methods:

    • Preparation of lung microsomes and 900 x g supernatant fractions from rats of different ages.
    • Assay of C-9 fatty acid desaturase activity.
    • Measurement of the incorporation of radiolabeled linoleate, linolenate, and arachidonate into triacylglycerols and phospholipids.

    Main Results:

    • C-9 fatty acid desaturase activity was present but lower in neonatal rat lungs compared to adults, increasing around weaning.
    • Neonatal rat lungs incorporated PUFA into phospholipids at a higher rate than into triacylglycerols.
    • Adult rat lungs preferentially incorporated PUFA into triacylglycerols over phospholipids.

    Conclusions:

    • Developmental changes in diet, particularly post-weaning, may influence lung fatty acid desaturase activity.
    • Neonatal rat lungs prioritize PUFA for essential phospholipid synthesis, crucial for membrane structure and function.
    • Adult rat lungs shift PUFA utilization towards triacylglycerol esterification, suggesting a role in energy storage or other functions.

    Related Experiment Videos