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Mutagenicity studies with the mouse spot test.

E Gocke, D Wild, K Eckhardt

    Mutation Research
    |April 1, 1983
    PubMed
    Summary
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    The mammalian spot test effectively detects gene mutations in mouse embryos. Ethylnitrosourea (ENU), ethyl methanesulfonate (EMS), and 2-acetylaminofluorene (2AAF) showed significant mutagenicity, validating the test system for environmental compounds.

    Area of Science:

    • Toxicology
    • Genetics
    • Mammalian Embryology

    Background:

    • The mammalian spot test is a system for detecting somatic gene mutations in mouse embryos.
    • Validation of this test system is crucial for assessing chemical mutagenicity.
    • Environmental compounds previously identified as mutagens require evaluation in a whole-mammal system.

    Purpose of the Study:

    • To further validate the mammalian spot test using known mutagens (ENU, EMS, 2AAF, colchicine).
    • To evaluate the mutagenic potential of environmental compounds in a whole-mammal system.
    • To assess compounds previously identified as mutagens in other test systems.

    Main Methods:

    • Application of selected chemicals to mouse embryos.
    • Observation and classification of coat color mutations (spots).

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  • Utilizing a fluorescence microscope for hair classification.

    • Main Results:

    • ENU, EMS, and 2AAF demonstrated significant mutagenicity in the mammalian spot test.
    • 1,2-dichloroethane exhibited probable weak mutagenicity.
    • Data from ENU treatment allowed estimation of pigment precursor cell numbers.

    Conclusions:

    • The mammalian spot test is validated as a reliable system for detecting somatic gene mutations.
    • The test system can effectively evaluate the mutagenic potential of environmental compounds.
    • Further research can utilize this system to assess various chemical agents and estimate cell populations.