Production of Kasahara isoenzyme by OKK cell line established from a human maxillary carcinoma

Area of Science:

• Biochemistry
• Cell Biology
• Oncology

Background:

• A novel human maxillary carcinoma cell line, designated OKK, was established from a Japanese patient.
• Tumorigenicity of the OKK cell line was confirmed by inoculation into nude mice.

Purpose of the Study:

• To biochemically and immunologically characterize the alkaline phosphatase isoenzymes from the OKK cell line and its derived tumors.
• To compare the properties of the OKK T isoenzyme with the Kasahara isoenzyme.

Main Methods:

• Partial purification of alkaline phosphatases using chromatography.
• Biochemical and immunological analyses of isoenzyme properties, including heat stability, amino acid inhibition, and molecular weight.
• Genetic phenotyping of glucose-6-phosphate dehydrogenase.

Main Results:

• The electrophoretically fastest alkaline phosphatase isoenzyme from OKK tumors (OKK T isoenzyme) was identified.
• The OKK T isoenzyme exhibited identical biochemical and immunological properties to the Kasahara isoenzyme.
• Key properties such as inhibition by L-phenylalanine and L-leucine, heat stability, immunological character, and molecular weight were consistent between OKK T and Kasahara isoenzymes.
• The glucose-6-phosphate dehydrogenase phenotype of the OKK cell line differed from that of the HeLa cell line.

Conclusions:

• The OKK T isoenzyme is biochemically and immunologically indistinguishable from the Kasahara isoenzyme.
• The established OKK cell line provides a valuable model for studying human maxillary carcinoma and its associated alkaline phosphatases.
• The distinct glucose-6-phosphate dehydrogenase phenotype differentiates the OKK cell line from HeLa cells.