Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complement activation by plasma separator membranes.

B C McLeod, A Viernes, R J Sassetti

    Transfusion
    |March 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Introduction to the third special issue: clinical applications of therapeutic apheresis.

    Journal of clinical apheresis·2000
    Same author

    Therapeutic apheresis in renal and metabolic diseases.

    Journal of clinical apheresis·2000
    Same author

    Frequency of immediate adverse effects associated with therapeutic apheresis.

    Transfusion·1999
    Same author

    Frequency of immediate adverse effects associated with apheresis donation.

    Transfusion·1998
    Same author

    Ex vivo expansion of frozen/thawed CD34+ cells isolated from frozen human apheresis products.

    Journal of hematotherapy·1997
    Same author

    Ultraviolet-B irradiation of leukapheresis. Products: dose-response relationship with the mixed lymphocyte reaction.

    Journal of clinical apheresis·1996
    Same journal

    Spray dried plasma manufactured from apheresis and whole blood derived plasma.

    Transfusion·2026
    Same journal

    Identification of a novel ABO*A1.01 allele with c.562C>T (p.Arg188Cys) mutation associated with A<sub>el</sub> phenotype in a Chinese individual.

    Transfusion·2026
    Same journal

    AABB survey on directed blood donation practices.

    Transfusion·2026
    Same journal

    Cost analysis considerations for red blood cell matching to mitigate alloimmunization in patients with sickle cell disease.

    Transfusion·2026
    Same journal

    A novel translation initiation codon single nucleotide variant of the SLC14A1 (c.2T>G) from a Chinese blood donor with Jk(a-b-) phenotype.

    Transfusion·2026
    Same journal

    Size (and frequency) matter: Evaluating the best management practices for blood products with spotty demand.

    Transfusion·2026
    See all related articles

    Six plasma separator membranes were tested for complement activation. Polysulfone and cellulose acetate membranes strongly activated the complement alternative pathway, while others showed weaker activation, informing material selection for plasma separators.

    Area of Science:

    • Biomaterials Science
    • Immunology
    • Medical Device Engineering

    Background:

    • Plasma separation membranes share structural similarities with dialysis membranes.
    • Complement activation by medical devices can lead to adverse immune responses.
    • Understanding membrane-induced complement activation is crucial for device safety and efficacy.

    Purpose of the Study:

    • To evaluate the complement-activating potential of six different plasma separator membranes.
    • To identify which membrane materials are strong or weak activators of the complement alternative pathway.
    • To assess the influence of anticoagulation on membrane-induced complement activation.

    Main Methods:

    • Crossed immunoelectrophoresis was used to detect complement activation via immunoconversion of the third component of complement (C3).

    Related Experiment Videos

  • Six distinct plasma separator membranes were tested, including polysulfone, cellulose acetate, polypropylene, poly(vinylidene fluoride), and polyvinyl chloride derivatives.
  • Plasma samples from different donors were used, with and without citrate or heparin anticoagulation.

    • Main Results:

    • Polysulfone and two cellulose acetate membranes demonstrated relatively strong activation of the complement alternative pathway.
    • Polypropylene, poly(vinylidene fluoride), and polyvinyl chloride derivative membranes exhibited weak activation in some sera.
    • Complement activation by membranes was inhibited when plasma was anticoagulated with citrate, but not with heparin.

    Conclusions:

    • Material choice for plasma separators significantly impacts complement activation.
    • Citrate anticoagulation effectively inhibits membrane-induced complement activation, unlike heparin.
    • In vitro screening of membrane materials and anticoagulation strategies is valuable for optimizing plasma separator safety and performance.