Adrenergic regulation of avian pancreatic polypeptide secretion in vitro

Adrenergic regulation of avian pancreatic polypeptide (APP) secretion from perifused microfragments of chicken pancreas was investigated with catecholamines and adrenoreceptor-specific analogues added to avian Krebs-Ringer perifusion medium containing 11.1 mM glucose. APP release was stimulated by the alpha-adrenoreceptor agonist phenylephrine (threshold 5 microM; maximum effective concn, 50 microM) and the beta-adrenoreceptor agonist isoproterenol (threshold 5 microM; maximum effective concn, 50 microM). Epinephrine (0.164 microM) and 50 microM norepinephrine also stimulated APP release. The results obtained suggest that APP secretion from chicken pancreas is regulated by dual, complementary alpha- and beta-adrenoreceptor mechanisms.