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Electron microscopic study on vaccinia virus release
Archives of Virology
|January 1, 1983
Summary
Vaccinia virus (VACV) infected cells showed naked viral particles accumulating under the plasma membrane and budding from the cell surface. These particles were also observed budding from cytoplasmic packets near the cell periphery.
Area of Science:
- Virology
- Cell Biology
- Microscopy
Background:
- Vaccinia virus (VACV) is a large, complex DNA virus.
- Understanding VACV replication and egress is crucial for vaccine development and antiviral strategies.
Purpose of the Study:
- To investigate the ultrastructural details of VACV assembly and release in FL cells.
- To characterize the sites and mechanisms of naked VACV particle egress.
Main Methods:
- Scanning electron microscopy (SEM) was used to examine the cell surface.
- Transmission electron microscopy (TEM) was employed to visualize intracellular and extracellular viral structures.
Main Results:
- Accumulation of numerous naked VACV particles beneath the host cell plasma membrane.
- Protrusion of naked viral particles from the cell surface.
- Observation of naked viral particles budding from both the cell surface and peripheral cytoplasmic packets.
Conclusions:
- Naked VACV particles egress via both cell surface budding and budding from cytoplasmic packets.
- These findings provide insights into the complex release mechanisms of vaccinia virus.