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Nuclear-hormone mediated changes in chromatin solubility.

R N Re, R A LaBiche, S E Bryan

    Biochemical and Biophysical Research Communications
    |January 14, 1983
    PubMed
    Summary
    This summary is machine-generated.

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    Thyroid hormone and angiotensin (AII) increase chromatin solubility in rat liver nuclei. This suggests hormones alter chromatin structure, making DNA more accessible to enzymes.

    Area of Science:

    • Molecular Biology
    • Endocrinology
    • Biochemistry

    Background:

    • Chromatin structure plays a crucial role in regulating DNA accessibility.
    • Hormonal signaling can influence nuclear processes and chromatin organization.

    Purpose of the Study:

    • To investigate the effect of thyroid hormone and angiotensin (AII) on rat liver chromatin solubility.
    • To determine if hormones alter DNA accessibility to nucleases.

    Main Methods:

    • Incubation of isolated rat liver nuclei with varying concentrations of thyroid hormone or AII.
    • Digestion of chromatin with micrococcal nuclease.
    • Isolation of nuclei from animals treated in-vivo with AII or buffer.

    Main Results:

    Related Experiment Videos

  • Hormone concentrations above 10(-10)M increased chromatin solubilization.
  • Physiological AII levels (10(-9)M) resulted in a 2.4-fold increase in solubility compared to controls.
  • In-vivo AII treatment also increased nuclear chromatin solubility, indicating endogenous nuclease involvement.

    • Conclusions:

    • Hormone-induced increases in chromatin solubility reflect structural modifications.
    • These structural changes enhance DNA accessibility for endonuclease activity.
    • Suggests a mechanism for hormonal regulation of gene accessibility.