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Updated: Aug 10, 2026

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen
Published on: September 20, 2012
Separation of native types I and III collagens and denatured chains by reverse-phase high-performance liquid
Abstract:
A simple and effective high-performance-liquid-chromatography system has been developed for the separation of the native forms of Types I and III collagens. This separation is achieved on a commercially available 330-A-pore-size C18 reverse-phase support using a solvent system consisting of ammonium bicarbonate (0.05 M) and trifluoroacetic acid (0.4%) with tetrahydrofuran as the eluting solvent. This simple system can also be applied to the separation of mixtures of the denatured chains of both collagen types.
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