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Age-related effect of cycloheximide on lipid peroxidation in rough and smooth microsomes of rat liver
Abstract:
Cycloheximide, at a nonlethal dose of 2 mg/kg body weight, has different effects on microsomal in vivo lipid peroxidation in different age groups. In general, the antibiotic increases microsomal lipid peroxidation in newborn and 1-year-old rats, but decreases it in 15- and 75-day-old rats. Low doses of cycloheximide induces lipid peroxidation in vitro whereas higher doses cause inhibition. The degree of change differs in the two microsomal fractions. The sucklings are more susceptible to such a change than the older rats. In vivo lipid peroxidation in rat liver homogenate and rough and smooth microsomes declines as a function of age. Smooth microsomes show more lipid peroxidation than the rough in the different age groups.
Insights
Cycloheximide affects lipid peroxidation differently in rats of various ages. This antibiotic increases peroxidation in newborns but decreases it in older rats, with varying effects on microsomal fractions.
Area of Science:
- Biochemistry
- Pharmacology
- Toxicology
Background:
- Lipid peroxidation is a key indicator of oxidative stress.
- Microsomal fractions play crucial roles in cellular metabolism and drug detoxification.
- Age-related changes in cellular processes can influence drug responses.
Purpose of the Study:
- To investigate the age-dependent effects of cycloheximide on in vivo lipid peroxidation.
- To compare the impact of cycloheximide on different microsomal fractions (rough and smooth).
- To explore the dose-dependent effects of cycloheximide on in vitro lipid peroxidation.
Main Methods:
- Administration of cycloheximide (2 mg/kg body weight) to rats of different age groups.
- Measurement of in vivo lipid peroxidation in liver homogenate and microsomal fractions.
- In vitro assessment of cycloheximide's effect on lipid peroxidation at varying concentrations.
Main Results:
- Cycloheximide increased in vivo lipid peroxidation in newborn and 1-year-old rats, but decreased it in 15- and 75-day-old rats.
- In vitro studies showed low doses of cycloheximide induced lipid peroxidation, while higher doses inhibited it.
- In vivo lipid peroxidation decreased with age in rat liver homogenates and microsomes, with smooth microsomes exhibiting higher peroxidation than rough microsomes.
Conclusions:
- The effect of cycloheximide on microsomal lipid peroxidation is age-dependent.
- Susceptibility to cycloheximide-induced changes in lipid peroxidation is higher in younger rats.
- Age influences basal levels of lipid peroxidation in rat liver microsomes, with smooth microsomes being more affected.