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Clinical applications of fluorometry.

L J Blanksma, A C Kooijman, J H Roze

Documenta Ophthalmologica. Advances in Ophthalmology

|February 28, 1983

Summary

A new instrument rapidly measures fluorescein in the eye, assessing blood-ocular barrier integrity. This helps differentiate conditions like uveitis and diabetic retinopathy by pinpointing barrier dysfunction.

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Area of Science:

Ophthalmology
Biomedical Engineering
Medical Diagnostics

Background:

The blood-ocular barriers, including the blood-aqueous and blood-retinal barriers, are crucial for maintaining ocular health.
Dysfunction of these barriers is implicated in various eye diseases, such as uveitis and diabetic retinopathy.
Accurate assessment of barrier integrity is essential for diagnosis and treatment monitoring.

Purpose of the Study:

To develop and validate a novel instrument for rapid, in-vivo measurement of fluorescein concentration in the eye.
To assess the utility of this instrument in evaluating the integrity of the blood-ocular barriers.
To differentiate the localization of barrier dysfunction in specific ocular conditions.

Main Methods:

Development of a non-invasive instrument capable of measuring fluorescein levels along a scanning line from retina to cornea.
Utilizing a spatial resolving power of 1.1 mm for precise measurements.
Detecting fluorescein concentrations as low as 1 x 10(-8) gr/ml.

Main Results:

The instrument provides measurements in ten seconds, offering a rapid assessment.
It demonstrates high sensitivity in detecting low fluorescein concentrations.
The method allows for the differentiation of barrier dysfunction, with uveitis affecting the blood-aqueous barrier and diabetic retinopathy primarily affecting the blood-retinal barrier.

Conclusions:

The developed instrument offers a valuable tool for assessing blood-ocular barrier integrity.
It enables non-invasive, rapid, and sensitive detection of fluorescein leakage.
This technology has the potential to aid in the differential diagnosis and management of ocular inflammatory and vascular diseases.

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