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Related Experiment Videos

Amines induce increased thromboplastin activity in human monocytes.

R T Dean, H Prydz

    European Journal of Biochemistry
    |April 5, 1983
    PubMed
    Summary

    Amines boost thromboplastin activity in human monocytes, requiring protein synthesis and peaking at 18 hours. This suggests amines may increase intracellular pH, impacting monocyte function.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Immunology

    Background:

    • Thromboplastin activity is crucial for blood coagulation.
    • Monocytes play a key role in immune responses and inflammation.
    • Intracellular pH regulation is vital for cellular functions.

    Purpose of the Study:

    • To investigate the effect of various amines on thromboplastin activity in human monocytes.
    • To determine the mechanism by which amines influence thromboplastin activity.
    • To explore the role of intracellular pH in amine-mediated effects on monocytes.

    Main Methods:

    • Primary human monocytes were cultured in vitro.
    • Monocytes were treated with a variety of amines at different concentrations and time points.
    • Thromboplastin activity was measured using a chromogenic assay.
    • Protein synthesis was assessed using radiolabeled amino acid incorporation.
    • Intracellular pH was monitored using pH-sensitive fluorescent probes.

    Main Results:

    • Amines induced a dose-dependent and time-dependent increase in monocyte thromboplastin activity.
    • The observed increase in thromboplastin activity was dependent on de novo protein synthesis.
    • Thromboplastin activity peaked approximately 18 hours after amine treatment.
    • Low concentrations of ammonium chloride (NH4Cl) exhibited inhibitory effects.
    • Evidence suggests that amines may increase the pH of acidic intracellular compartments.

    Conclusions:

    • Amines significantly enhance thromboplastin activity in human monocytes.
    • The effect of amines on thromboplastin activity is mediated by protein synthesis.
    • Amines likely exert their effects by modulating intracellular pH within acidic compartments.

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