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Related Experiment Videos

Vesicular diffusion and thermal forces.

S M Shea, J Raskova

    Federation Proceedings
    |May 15, 1983
    PubMed
    Summary
    This summary is machine-generated.

    The endothelial vesicular shuttle theory explains macromolecular transport in microcirculation. New fixation methods are needed to reconcile conflicting evidence on vesicle presence in capillary endothelium.

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    Area of Science:

    • Cell biology
    • Physiology
    • Microcirculation research

    Background:

    • The endothelial vesicular shuttle theory proposes vesicles facilitate macromolecular transport across microcirculation.
    • Morphological studies using tracers and electron microscopy have investigated plasmalemmal vesicles for two decades.
    • Theoretical models suggest vesicular transport times are on the order of seconds.

    Purpose of the Study:

    • To review theoretical models of vesicular transport.
    • To discuss recent experimental evidence and its implications for the vesicular shuttle theory.
    • To highlight discrepancies in fixation methods and their impact on understanding endothelial structure.

    Main Methods:

    • Review of theoretical models of vesicular transport, including diffusion-based and hydrodynamically constrained models.

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  • Analysis of experimental evidence from tracer studies and electron microscopy.
  • Comparison of findings from aldehyde fixation versus potential instantaneous fixation methods.

    • Main Results:

    • Theoretical models predict vesicular transport times in the order of seconds.
    • Recent experimental data partially support these predictions.
    • Aldehyde fixation reveals few free vesicles, contradicting in vivo tracer studies and the shuttle theory.

    Conclusions:

    • Aldehyde fixation may not accurately represent the in vivo state of capillary endothelium.
    • Contradictory findings suggest a need for more instantaneous fixation techniques.
    • Rapid freezing combined with tracer studies and serial sectioning may resolve current contradictions in vesicular transport research.