Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Determination of prothrombin by a micro-coagulation method.

B Kirchhof, S Schmale, J van de Loo

|January 1, 1983

Summary

This study introduces a novel two-step assay for accurate prothrombin determination, differentiating between carboxy- and decarboxyprothrombin. This method offers improved specificity for prothrombin measurement in coagulation diagnostics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Unilateral recurrent elevation of intraocular pressure with hypopyon].

Die Ophthalmologie·2021

Same author

Alteration of MCP-1 and MMP-9 in Aqueous Humor Is Associated with Secondary Glaucoma in Fuchs Uveitis Syndrome.

Ocular immunology and inflammation·2019

Same author

[Laser flare photometry for identification of high-risk patients for proliferative vitreoretinopathy].

Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2018

Same author

[Watchful waiting as a therapeutic principle for diseases of the vitreoretinal interface].

Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2017

Same author

[Vision Loss after Silicone Oil Surgery].

Klinische Monatsblatter fur Augenheilkunde·2017

Same author

Spectral-domain optical coherence tomography findings of the macula in 500 consecutive patients with uveitis.

Eye (London, England)·2016

Area of Science:

Biochemistry
Hematology
Clinical Chemistry

Background:

Prothrombin is a crucial vitamin K-dependent protein in the coagulation cascade.
Accurate measurement of prothrombin levels is essential for diagnosing and managing bleeding disorders.
Existing assays may lack specificity or be influenced by other coagulation factors.

Purpose of the Study:

To develop and validate a novel two-step assay for precise prothrombin determination.
To differentiate between functional (carboxyprothrombin) and non-functional (decarboxyprothrombin) forms of prothrombin.
To assess the performance of the new assay against established coagulation tests.

Main Methods:

A two-step reaction involving prothrombin activation by human factor Xa, phospholipids, Ca++, and factor V.
Assay of activated prothrombin via fibrinogen proteolysis.
Differentiation of prothrombin forms using Echis carinatus venom as an alternative activator.
Comparison with conventional one-stage coagulation tests, chromogenic substrate assays, and Laurell electrophoresis.

Main Results:

The developed assay is specific for prothrombin, independent of other thrombin generation components.
The assay successfully differentiates between carboxyprothrombin and decarboxyprothrombin.
The micro-coagulation assay demonstrates comparable or superior performance to conventional methods.

Conclusions:

The novel two-step assay provides a specific and reliable method for prothrombin quantification.
This assay enables the distinction between functional and non-functional prothrombin, aiding in clinical diagnosis.
The assay represents a significant advancement in prothrombin measurement for coagulation diagnostics.

Related Experiment Videos