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Related Experiment Videos

Corneal re-epithelialization in galactosemic rats.

M B Datiles, P F Kador, H N Fukui

    Investigative Ophthalmology & Visual Science
    |May 1, 1983
    PubMed
    Summary
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    Aldose reductase (AR) inhibitors prevent corneal healing defects in diabetic and galactosemic rats. This suggests AR plays a key role in impaired corneal re-epithelialization associated with diabetes.

    Area of Science:

    • Ophthalmology
    • Diabetic Complications
    • Corneal Biology

    Background:

    • Corneal epithelial healing abnormalities are noted in diabetic patients.
    • Defects in corneal re-epithelialization in diabetic rats are preventable with aldose reductase (AR) inhibitors.

    Purpose of the Study:

    • To investigate the role of aldose reductase (AR) in corneal re-epithelialization defects using galactosemic rats.
    • To determine if AR is a common factor in sugar-induced corneal healing issues.

    Main Methods:

    • Galactosemic rat model induced to mimic diabetic conditions.
    • Computer planimetry to quantify re-epithelialization delay.
    • Light and scanning electron microscopy to assess corneal structure and cell migration.
    • Treatment with aldose reductase inhibitor (Sorbinil).

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    Main Results:

    • Galactosemic rats exhibited similar corneal re-epithelialization defects as diabetic rats.
    • Microscopy revealed corneal edema and wider intercellular spaces.
    • Reduced filopodia formation was observed during epithelial cell migration.
    • Sorbinil treatment prevented these observed defects.

    Conclusions:

    • Aldose reductase (AR) plays a significant role in the corneal re-epithelialization defects seen in diabetes.
    • Targeting AR may offer a therapeutic strategy for diabetic corneal complications.