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Related Experiment Videos

Collaborative evaluation of the microbial profile system for quantitative antimicrobial susceptibility testing.

E H Gerlach, R N Jones, A L Barry

    Journal of Clinical Microbiology
    |March 1, 1983
    PubMed
    Summary

    The Microbial Profile System (MPS) antimicrobial panels demonstrated high agreement with reference methods across multiple studies. This antimicrobial susceptibility testing system is reliable for clinical isolates and resistant strains.

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    Area of Science:

    • Microbiology
    • Clinical Diagnostics
    • Antimicrobial Resistance

    Background:

    • Accurate antimicrobial susceptibility testing (AST) is crucial for guiding effective treatment of bacterial infections.
    • Microdilution methods are standard for determining minimum inhibitory concentrations (MICs).
    • Evaluating novel AST systems against established methods is essential for clinical adoption.

    Purpose of the Study:

    • To assess the performance and reliability of the Microbial Profile System (MPS) antimicrobial microdilution panels.
    • To compare MPS panel results with a reference microdilution method across various bacterial strains and study phases.

    Main Methods:

    • A three-center collaborative study involving three phases.
    • Phase I: Assessed inter- and intralaboratory agreement using strains with known susceptibility ranges.

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  • Phase II: Evaluated performance with strains exhibiting known resistance mechanisms and tested 359 clinical isolates.

    • Main Results:

    • High intralaboratory agreement (97.7% for MPS, 98.8% for reference) and interlaboratory agreement (96.2% for MPS, 96.0% for reference) were observed.
    • Overall agreement for strains with known resistance mechanisms was 94.7%, with specific high percentages for Enterobacteriaceae (94.5%), nonenteric gram-negative rods (95.4%), staphylococci (92.3%), and streptococci (96.6%).
    • Testing 359 clinical isolates yielded 97.3% agreement between MPS and the reference method.

    Conclusions:

    • The Microbial Profile System (MPS) antimicrobial microdilution panels demonstrated performance equivalent to the reference microdilution method.
    • MPS panels provide reliable and accurate results for a broad spectrum of bacteria, including resistant strains and clinical isolates.
    • The study supports the clinical utility of the MPS for antimicrobial susceptibility testing.