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Scanning electron microscope studies on preparations of bovine cornea exposed to Moraxella bovis
Journal of Comparative Pathology
|January 1, 1983
Abstract:
Preparations of bovine cornea were studied by scanning electron microscopy before and after in vitro exposure to Moraxella bovis. The bacteria were observed in association with the surface of the corneal epithelium; they showed a predilection for dark cells relatively devoid of surface structure. Pit-like depressions related to the presence of individual bacteria were produced in these cells. An evident association with corneal epithelium was shown by 2 strains known to be pathogenic experimentally in cattle but not by 2 non-pathogenic strains.
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01:07Scanning Electron Microscopy
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
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Fundamental Principles
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01:22Overview of Microscopy Techniques
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...