Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Analysis of rabbit beta-like globin gene transcripts during development.

M L Rohrbaugh, R C Hardison

Journal of Molecular Biology

|March 5, 1983

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Exponential decay of GC content detected by strand-symmetric substitution rates influences the evolution of isochore structure.

Molecular biology and evolution·2007

Same author

Global predictions and tests of erythroid regulatory regions.

Cold Spring Harbor symposia on quantitative biology·2004

Same author

Association between divergence and interspersed repeats in mammalian noncoding genomic DNA.

Proceedings of the National Academy of Sciences of the United States of America·2001

Same author

Databases of human hemoglobin variants and other resources at the globin gene server.

Hemoglobin·2001

Same author

Sequences flanking hypersensitive sites of the beta-globin locus control region are required for synergistic enhancement.

Molecular and cellular biology·2001

Same author

New views of evolution and regulation of vertebrate beta-like globin gene clusters from an orphaned gene in marsupials.

Proceedings of the National Academy of Sciences of the United States of America·2001

Same journal

UPF3A and UPF3B shape the transcriptome cooperatively yet oppose cell function.

Journal of molecular biology·2026

Same journal

Antibody-secreting cells integrate efficient NMD with non‑canonical UPR signaling to maintain proteostasis and support massive immunoglobulin synthesis.

Journal of molecular biology·2026

Same journal

Small molecule stabilization of diverse amyloidogenic immunoglobulin light chains revealed by hydrogen-deuterium exchange mass spectrometry.

Journal of molecular biology·2026

Same journal

UPF1 at Work: Structural and Mechanistic Insights Into a Master Regulator of Nonsense-Mediated mRNA Decay.

Journal of molecular biology·2026

Same journal

Structural basis for the pro-amyloidogenic action and ligand binding of a novel W72R variant of human apolipoprotein A-I.

Journal of molecular biology·2026

Same journal

Cryo-EM Structure of the C. Elegans Septin Tetramer Reveals a Revised Architecture and Conserved Positional Orthology.

Journal of molecular biology·2026

See all related articles

Rabbit beta-like globin gene expression shifts from embryonic (beta 3, beta 4) to adult (beta 1) forms during development. This developmental switch in globin gene regulation occurs primarily at the transcriptional level.

Area of Science:

Developmental Biology
Molecular Genetics
Hematopoiesis

Background:

Globin gene families undergo developmental regulation.
Rabbit beta-like globin genes include beta 4, beta 3, pseudogene beta 2, and beta 1.
Erythropoiesis shifts from yolk sac to fetal liver during rabbit gestation.

Purpose of the Study:

To analyze differential expression of rabbit beta-like globin genes during development.
To identify embryo-specific globin polypeptides.
To determine the regulatory mechanisms controlling globin gene switching.

Main Methods:

Hybrid-arrested translation in vitro to identify globin polypeptides.
Blot-hybridization and S1 nuclease protection assays to analyze RNA transcripts.

Related Experiment Videos

Analysis of gene expression from four days post-implantation to one week before birth.

Main Results:

Embryo-specific beta 3 and beta 4 globin polypeptides were identified.
Adult beta 1 globin replaced embryonic beta 3 and beta 4 globins mid-gestation.
Gene expression analysis revealed coordinate regulation of precursor RNA, mRNA, and polypeptide production.
Embryonic globin genes (beta 3, beta 4) are transcribed in early development, while adult globin gene (beta 1) is transcribed later.

Conclusions:

Developmental regulation of rabbit beta-like globin genes is primarily at the transcriptional level.
The switch from embryonic to adult globin expression is tightly coordinated.
Formaldehyde-induced artifacts in RNA analysis were identified and characterized.