Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium and fibrin gel structure.

M Okada, B Blombäck

    Thrombosis Research
    |February 1, 1983
    PubMed
    Summary

    Calcium ions (Ca) significantly impact fibrin gel structure and liquid permeability during formation. While essential for gelation, calcium

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Travels with fibrinogen.

    Journal of thrombosis and haemostasis : JTH·2006
    Same author

    Fibrinogen: evolution of the structure-function concept. Keynote address at fibrinogen 2000 congress.

    Annals of the New York Academy of Sciences·2001
    Same author

    Fibrin formation in whole blood.

    Thrombosis research·2001
    Same author

    Thrombosis research 30 years.

    Thrombosis research·2000
    Same author

    In memoriam, Per Wallén, 1927-1999.

    Thrombosis research·1999
    Same author

    Characterization of a fibrin glue-GDNF slow-release preparation.

    Cell transplantation·1998

    Area of Science:

    • Biochemistry
    • Biophysics
    • Materials Science

    Background:

    • Fibrin gels are crucial in hemostasis and wound healing.
    • Calcium ions (Ca) are known to play a role in blood coagulation.
    • The precise influence of Ca on fibrin gel structure and properties requires further elucidation.

    Purpose of the Study:

    • To investigate the effect of calcium ions on the formation and properties of fibrin gels.
    • To determine the role of Ca in fibrin gelation kinetics and liquid permeability.
    • To compare the Ca dependence of Fibrin I and Fibrin II gels.

    Main Methods:

    • Formation of Fibrin I and Fibrin II gels at varying Ca concentrations.
    • Measurement of gel permeability coefficients (Ks) and clotting times (Ct).
    • Assessment of fibrinogen activation and the effect of Ca removal using EDTA.

    Main Results:

    • Increasing Ca concentration enhanced liquid permeation (Ks) of fibrin gels, reaching a maximum at 10-20 mM.
    • Calcium shortened clotting times (Ct) and decreased the activation required for gelation.
    • Magnesium shortened Ct but did not affect Ks; Ca removal post-formation did not alter flow properties.

    Conclusions:

    • Calcium ions are critical for the formation of fibrin gel structure, influencing its permeability.
    • Calcium's role is more significant during fibrin gel formation than in preserving the established structure.
    • Fibrin I and Fibrin II gels exhibit distinct dependencies on calcium during their formation.

    Related Experiment Videos