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Related Experiment Videos

A simple cell disrupter designed for small cells with relatively large nuclei.

S Szuchet, P E Polak

    Analytical Biochemistry
    |February 1, 1983
    PubMed
    Summary

    A novel cell disrupter efficiently breaks small cells with large nuclei using nitrogen pressure and microfilters. This rapid, reproducible method yields high-quality homogenates for isolating cellular components.

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    Molecular and cellular biology·2001

    Area of Science:

    • Cell biology
    • Biophysical techniques

    Background:

    • Efficient disruption of small cells, especially those with large nuclei, is crucial for biological research.
    • Existing methods may be time-consuming or yield suboptimal results for specific cell types.

    Purpose of the Study:

    • To describe a simple, rapid, and reproducible method for disrupting small cells.
    • To present a cell disrupter suitable for isolating plasma membranes, organelles, and nuclei.

    Main Methods:

    • Utilizing shearing forces generated by pushing cells through a 0.8- to 1.5-micrometer stainless-steel filter.
    • Employing positive nitrogen (N2) pressure for cell propulsion.

    Main Results:

    • The procedure is completed within minutes.
    • The method demonstrates high reproducibility and good yield.
    • The resulting cell homogenate is suitable for further molecular analysis.

    Conclusions:

    • The described cell disrupter offers an effective solution for lysing small cells with large nuclei.
    • This technique facilitates the efficient isolation of key cellular components, including plasma membranes, intracellular organelles, and nuclei.

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