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Related Experiment Videos

Differences in polyamine availability and insertion into fibronectins released from normal and transformed cells.

A M Roch, G Quash, H Ripoll

    The Biochemical Journal
    |January 15, 1983
    PubMed
    Summary

    Transformed kidney cells release fibronectin with increased amino groups, affecting polyamine insertion. This suggests structural changes in fibronectin from cancer cells, impacting biological interactions.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Proteomics

    Background:

    • Fibronectin is a crucial extracellular matrix protein involved in cell adhesion, migration, and differentiation.
    • Alterations in fibronectin structure and function are associated with cellular transformation and cancer progression.

    Purpose of the Study:

    • To investigate biochemical differences in fibronectin released from normal versus transformed rat kidney cells.
    • To explore the impact of cellular transformation on fibronectin's interaction with polyamines and its secondary structure.

    Main Methods:

    • Comparative analysis of amino, thiol, and carboxy group availability in fibronectin from normal and transformed cells.
    • Assay of polyamine content and transglutaminase-mediated polyamine insertion kinetics.
    • Immunochemical analysis using specific antisera.

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    Main Results:

    • Fibronectin from transformed cells exhibited a 50% increase in amino group availability compared to normal cells.
    • Transglutaminase-mediated spermidine insertion showed substrate inhibition kinetics with transformed cell fibronectin, unlike linear kinetics with normal fibronectin.
    • Immunochemical analyses revealed distinct reactivity patterns between normal and transformed fibronectins.

    Conclusions:

    • Transformed rat kidney cells release fibronectin with altered biochemical properties, specifically increased amino group availability.
    • These alterations suggest changes in fibronectin's secondary structure and its interaction with polyamines, potentially contributing to altered cellular behavior in transformed cells.