Fucosyl-globoside and sialosyl-globoside are new glycolipids isolated from human teratocarcinoma cells

Several novel glycosphingolipids have been isolated from the human teratocarcinoma cell line HT-E (833K). These cells contain two neutral glycolipids which metabolically incorporate radio-labelled fucose. In addition, there are three new gangliosides present, which are all members of the globoside series of glycolipids. One of the fucose containing glycolipids forms globoside when treated with alpha-fucosidase, and one of the gangliosides forms globoside when treated with neuraminidase. On the basis of chromatographic behavior, exoglycosidase treatment and antibody reactivity, the tentative structures of these new glycolipids are: Fuc (alpha 1-?) GalNAc (beta 1-3) Gal (alpha 1-4) Gal (beta 1-4) Glc (beta 1-Cer) and NeuAc (alpha 2-3) GalNAc (beta 1-3) Gal (alpha 1-4) Gal (beta 1-4) Glc (beta 1-Cer)