Leukocytes from patients allergic to chromium and nickel examined by the sealed capillary migration technique

Leukocytes from patients allergic to chromium and nickel were studied by a sealed capillary migration test. The migration indices were determined at 2, 5 and 24 h. The chromium-allergic group could be differentiated from the control group at all investigated times, and especially at 2 and 5 h, by using a potassium dichromate concentration of 1.7 x 10(-5) M in the capillaries. The migration indices in the nickel-allergic group could not be used for discrimination from the controls. The highest chromate (1.1 x 10(-4) M) and nickel sulfate (3.8 x 10(-4) M0 concentrations inhibited the migration of leukocytes from both patients and controls, indicating toxic effects.