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[Use of a histological fixative for extraction of the RNA precursor pool from mollusk nerve tissue]
Tsitologiia
|April 1, 1978
Abstract:
The incorporation of 3H-uridine into the nucleotide pool of the molluscan nerve tissue was calculated in a routine fixative solution (formalin--alcohol--acetic acid, 9:3:1). The optimal conditions for the precursor output from the tissue being studied, the results obtained well compare with those obtained by common biochemical techniques. Our method gives a possibility to use the same sample of tissue for historadiographical investigation of labeled RNA and for cytochemical determination of nuclear acid amounts.