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Effect of Konjac mannan on 1,2-dimethylhydrazine-induced intestinal carcinogenesis in Fischer 344 rats
Abstract:
The effect of Konjac mannan (KM) on 1,2-dimethylhydrazine (DMH-induced intestinal carcinogenesis was studied in male F344 rats. Rats were fed a diet containing 5% KM at 5 weeks of age. At 6 weeks of age, all animals were given a weekly intraperitoneal injection of 20 mg DMH/kg body wt for 13 weeks and autopsied 13 weeks after the last injection of DMH. The weight gain was lower in rats fed the KM diet than in rats fed the control diet throughout the experiment (P less than 0.05). The incidence of DMH-induced colon tumors was lower in animals fed the KM diet compared to animals fed the control diet (P less than 0.05). The number of colon adenocarcinoma per animal was also lower in animals fed the KM than in animals fed the control diet (P less than 0.05). However, the incidence of tumors of the small intestine did not significantly differ between the groups fed the KM and control diets. The present study demonstrated that colon tumorigenesis induced by DMH in F344 rat was inhibited by maintaining the KM diet.
Insights
Konjac mannan (KM) significantly reduced 1,2-dimethylhydrazine (DMH)-induced colon tumors in rats. This dietary fiber shows potential for preventing colon cancer development.
Area of Science:
- Gastroenterology
- Oncology
- Nutritional Science
Background:
- Intestinal carcinogenesis is a significant health concern.
- Dietary factors play a crucial role in cancer development and prevention.
- 1,2-dimethylhydrazine (DMH) is a chemical inducer of experimental intestinal cancer.
Purpose of the Study:
- To investigate the chemopreventive effect of Konjac mannan (KM) on DMH-induced intestinal carcinogenesis in a rat model.
- To evaluate the impact of KM on tumor incidence, multiplicity, and location.
Main Methods:
- Male F344 rats were fed a diet containing 5% KM starting at 5 weeks of age.
- DMH was administered intraperitoneally weekly for 13 weeks to induce intestinal tumors.
- Tumor development was assessed 13 weeks after the final DMH injection.
Main Results:
- Rats fed the KM diet exhibited lower body weight gain compared to controls.
- The incidence and number of colon adenocarcinomas were significantly reduced in the KM-fed group.
- No significant difference in small intestine tumor incidence was observed between KM and control groups.
Conclusions:
- Konjac mannan (KM) demonstrates a significant inhibitory effect on DMH-induced colon tumorigenesis in rats.
- Dietary KM may serve as a potential chemopreventive agent against colon cancer.
- Further research is warranted to explore the mechanisms and human applicability.