Association of cholesterol with lysophosphatidylcholine

With equimolar cholesterol, lysophosphatidylcholine (lysoPC) or 1-ether-2-deoxylyso-phosphatidylcholine (etherdeoxylysoPC) form unilamellar vesicles of identical dimensions. 13C-NMR spectra of such vesicles are interpreted on the premise that suppression of a signal by broadening (i.e. decrease of T*2 relaxation time) indicates a decrease of motion of the carbon atom relative to its surroundings. The signals for sn-glycerol C-1 and C-2 are completely suppressed in the lysoPC-cholesterol vesicles. In contrast, in the vesicles containing etherdeoxylysoPC, all three glycerol carbon signals make their appearance, with the T*2 of C-2 approaching the T*2 in the monomolecularly dissolved lysolipid. This result argues for lipid-lipid complexing in the "hydrogen belts' of the lysoPC-cholesterol bilayer, specifically, for hydrogen bonding involving the hydroxyl and carbonyl groups of lysoPC and the hydroxyl of cholesterol.