Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Flow sorting in the study of cell-cell interaction.

G H Schaap, A Verkerk, J Vijg

    Cytometry
    |May 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Within patient horizontal gene transfer dynamics of a bla<sub>NDM-7</sub> plasmid among four different bacterial species.

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2025
    Same author

    Single-cell analysis reveals different age-related somatic mutation profiles between stem and differentiated cells in human liver.

    Science advances·2020
    Same author

    Restricted diet delays accelerated ageing and genomic stress in DNA-repair-deficient mice.

    Nature·2016
    Same author

    5-aza-2'-deoxycytidine-induced genome rearrangements are mediated by DNMT1.

    Oncogene·2012
    Same author

    Genome dynamics and transcriptional deregulation in aging.

    Neuroscience·2006
    Same author

    Rapid, inexpensive scanning for all possible BRCA1 and BRCA2 gene sequence variants in a single assay: implications for genetic testing.

    Journal of medical genetics·2003

    This study investigated if mouse teratocarcinoma cells differentiate when cultured with endoderm cells. Results showed no differentiation, indicating teratocarcinoma cell differentiation was not induced by endoderm cell contact.

    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Cancer Research

    Background:

    • Teratocarcinoma cells are undifferentiated cells with pluripotency.
    • Studying cell-cell interactions is crucial for understanding differentiation induction.

    Purpose of the Study:

    • To investigate the potential of differentiated mouse endoderm cells to induce differentiation in undifferentiated mouse teratocarcinoma cells.
    • To develop a method for isolating and analyzing teratocarcinoma cells after co-cultivation.

    Main Methods:

    • Generating pseudotetraploid (2n) endoderm cell lines from pseudodiploid (1n) cells using cytochalasin B and flow sorting.
    • Co-cultivating teratocarcinoma cells with endoderm cells and re-isolating teratocarcinoma cells using DNA content differences.
    • Analyzing protein patterns using two-dimensional electrophoresis to assess differentiation.

    Related Experiment Videos

    Main Results:

    • Teratocarcinoma cells were successfully re-isolated from co-cultivation mixtures with high purity (95-97%).
    • Two-dimensional protein analysis did not reveal an endoderm-specific differentiation pattern in the re-isolated teratocarcinoma cells.
    • No evidence of induced differentiation in teratocarcinoma cells after 24 days of co-cultivation.

    Conclusions:

    • Co-cultivation with differentiated mouse endoderm cells did not induce differentiation in mouse teratocarcinoma cells.
    • The developed method of using DNA content differences is effective for studying cell-cell interactions in vitro.
    • Further research is needed to explore other factors influencing teratocarcinoma cell differentiation.