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Urease activity in Trichophyton mentagrophytes.

A K Sanyal, A B Banerjee

    Folia Microbiologica
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Urease activity was found in Trichophyton mentagrophytes during early growth but decreased with age. High urea levels inhibited urease formation, with optimal activity at pH 7-7.5.

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    Area of Science:

    • Mycology
    • Enzymology
    • Dermatophyte Research

    Background:

    • Urease enzymes are crucial in microbial metabolism and pathogenesis.
    • Dermatophytes, like Trichophyton species, are fungi causing skin infections.
    • Understanding urease activity in dermatophytes can offer insights into their growth and survival.

    Purpose of the Study:

    • To investigate the presence and characteristics of urease activity in Trichophyton mentagrophytes.
    • To determine the influence of culture age and urea concentration on urease production.
    • To compare urease activity between T. mentagrophytes and T. rubrum.

    Main Methods:

    • Culturing Trichophyton mentagrophytes and Trichophyton rubrum under varying conditions.
    • Measuring urease activity in cellular extracts and cell-free culture fluid.

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  • Assessing the effect of different exogenous urea concentrations on enzyme formation.
  • Determining the pH optimum and Michaelis constant (Km) for urease activity.

    • Main Results:

    • Urease activity was detected in T. mentagrophytes cells during the early exponential growth phase.
    • Specific urease activity diminished as the culture aged.
    • Urease formation was inhibited at exogenous urea concentrations exceeding 2 mM.
    • The optimal pH for urease activity was between 7 and 7.5, with a Km of 14 mM.
    • No urease activity was found in cell-free culture fluid of T. mentagrophytes or in any T. rubrum samples.

    Conclusions:

    • Urease in T. mentagrophytes is primarily intracellular and linked to early growth stages.
    • The fungus exhibits regulatory mechanisms for urease production in response to urea availability.
    • T. rubrum appears to lack detectable urease activity under the tested conditions.