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Related Experiment Videos

New initiation factor activity required for globin mRNA translation.

J A Grifo, S M Tahara, M A Morgan

    The Journal of Biological Chemistry
    |May 10, 1983
    PubMed
    Summary
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    Researchers identified a new protein complex, eukaryotic initiation factor 4F (eIF-4F), essential for maximal globin synthesis. This complex, containing a cap-binding protein, restores capped mRNA translation in viral-infected cells.

    Area of Science:

    • Molecular Biology
    • Protein Biochemistry
    • Gene Expression Regulation

    Background:

    • Protein synthesis is crucial for cellular function.
    • Eukaryotic translation initiation involves complex regulatory factors.
    • Specific initiation factors are required for efficient mRNA translation.

    Purpose of the Study:

    • To identify novel factors involved in rabbit globin synthesis.
    • To characterize a newly discovered activity stimulating translation.
    • To investigate the role of protein complexes in capped mRNA translation.

    Main Methods:

    • Utilized a reconstituted reticulocyte translation system deficient in eukaryotic initiation factor 4B (eIF-4B).
    • Employed various chromatographic procedures for protein complex purification.

    Related Experiment Videos

  • Analyzed protein components using gel electrophoresis and cross-linking assays.
  • Tested the activity of purified complexes in translation assays.

    • Main Results:

    • Identified and purified a high molecular weight complex termed eukaryotic initiation factor 4F (eIF-4F).
    • Demonstrated that eIF-4F stimulates maximal rabbit globin synthesis.
    • Showed eIF-4F restores translation of capped mRNAs in poliovirus-infected HeLa cell extracts.
    • Characterized eIF-4F as containing a 24,000-dalton cap binding protein and other polypeptides.

    Conclusions:

    • eIF-4F is a novel and essential factor for efficient translation initiation.
    • The cap binding protein within eIF-4F plays a critical role in translation.
    • eIF-4F activity is conserved and functional in viral mRNA translation.