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Pyridoxal phosphate as an antisickling agent in vitro
The Journal of Clinical Investigation
|May 1, 1983
Summary
Pyridoxal phosphate and pyridoxal inhibit sickle cell sickling in intact erythrocytes. Pyridoxal phosphate
Area of Science:
- Hematology
- Biochemistry
- Molecular Biology
Background:
- Pyridoxal phosphate inhibits purified hemoglobin S gelation.
- Antisickling activity in intact erythrocytes has not been previously demonstrated.
Purpose of the Study:
- To investigate the antisickling activity of pyridoxal phosphate and pyridoxal in intact erythrocytes.
- To compare their effects on hemoglobin modification, oxygen affinity, and sickling.
Main Methods:
- Washed erythrocytes were incubated with pyridoxal phosphate or pyridoxal.
- Hemoglobin modification, oxygen affinity, and sickling extent under hypoxia were measured.
Main Results:
- Pyridoxal phosphate modified hemoglobin slower than pyridoxal.
- Pyridoxal phosphate inhibited sickling significantly, largely independent of oxygen binding.
- Pyridoxal inhibited sickling, primarily dependent on increased oxygen binding.
Conclusions:
- Both pyridoxal phosphate and pyridoxal demonstrate antisickling activity in intact erythrocytes.
- They differ in modification kinetics, effects on oxygen affinity, and mechanism of action.
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