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[Proline arylamidase in human serum].

W Appel

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |January 1, 1983
    PubMed
    Summary
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    This study optimized a continuous assay for proline arylamidase in human serum, establishing normal ranges and suggesting its diagnostic potential. The assay is reliable for measuring enzyme activity in patient samples.

    Area of Science:

    • Biochemistry
    • Enzymology

    Context:

    • Proline arylamidase activity in human serum is a marker of potential diagnostic value.
    • Accurate and reliable measurement of enzyme activity is crucial for clinical applications.

    Purpose:

    • To optimize a continuous reaction method for determining proline arylamidase catalytic concentrations in human sera.
    • To establish the optimal conditions and assess the reliability of the assay.
    • To investigate the diagnostic potential of proline arylamidase in patient sera.

    Summary:

    • A continuous assay using L-proline-p-nitranilide was optimized for proline arylamidase in human serum (pH 7.2, 37°C).
    • The assay showed minimal interference from various substances, with EDTA and benzethonium chloride causing inhibition.
    • Normal catalytic concentrations were below 10 U/l, with a preliminary upper limit of 8.0 U/l, and demonstrated good reproducibility.

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  • Comparative analysis with other amino acid substrates suggests a specific diagnostic role for proline arylamidase.

    • Impact:

    • Provides a validated, optimized continuous assay for proline arylamidase in human serum.
    • Establishes reference ranges for enzyme activity, aiding in clinical interpretation.
    • Highlights the potential diagnostic utility of proline arylamidase in various patient conditions.