Bile restitution procedures for studying bile secretion in fistulated pigs

In order to study bile secretion in the fistulated pig, an apparatus which reinfused the secreted bile at a rate reflecting the secretion rate, was used. The essential parts were a glass container equipped with two platinum wire electrodes, an electronic relay, a stop valve, and a peristaltic pump. This apparatus allowed a reinfusion of collected bile in a volume determined by the position of one of the electrodes, the recording of bile flow, and a continuous sampling for chemical analysis.