Oestradiol induced changes of soluble protein synthesis in human endometrium

The effect of an in vitro application of oestradiol-17-beta on the soluble protein synthesis in human endometrium during the menstrual cycle was investigated. Results obtained by double-label technique and SDS-polyacrylamide gel or Cellogel electrophoresis show that synthesis of soluble proteins in human endometrium is affected by oestradiol in both follicular and luteal phases of the cycle. One of the proteins obtained after oestradiol treatment seems to be similar to IP of rat uterus as judged from its molecular weight (41,000 daltons) and electrophoretic mobility (RF:1.18-1.24 relative to BSA on Cellogel). The magnitude of this protein synthesis correlates significantly with cytoplasmic oestradiol receptor concentration in endometrium throughout the cycle. The highest induction of IP like protein(s) synthesis at follicular phase and a decreased induction during the luteal phase was detected. No quantitative relationship between the changes of progesterone receptor concentration and IP like protein synthesis was found.