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Morphology of experimental actinomycotic abscess in mice with Dermatophilus-like microorganisms from porcine tonsil
Abstract:
Experimental infection in mice with Dermatophilus (D.) congolensis-like microorganisms was carried out, intraperitoneally and subcutaneously. This strain had been isolated from porcine tonsil and reported to be different in some morphological and biological points from D. congolensis. Macroscopic examination revealed multiple abscesses in the peritoneal cavities, or subcutaneous abscesses after the intraperitoneal or subcutaneous injection respectively. Histopathologic examination revealed the characteristic arrangement of the neutrophils surrounding the bacterial colony and peripheral macrophages in the abscess lesions. The lesions contained many microorganisms which showed wide range of the characteristic morphologic variation such as: mycelial elements, coccoid elements and large coccoid elements with transverse or longitudinal septa. Chlamydospore-like elements were sometimes found in the microcolonies in early lesions. The morphology of the lesions and the microorganisms was compared with those of other bacteria including D. congolensis.
Insights
Experimental infection of mice with a Dermatophilus congolensis-like microorganism, isolated from pigs, caused abscesses. Histopathology revealed characteristic lesions and diverse microbial morphologies, differing from D. congolensis.
Area of Science:
- Veterinary Microbiology
- Pathology
- Infectious Diseases
Background:
- Dermatophilus congolensis causes skin infections in animals.
- A related microorganism, isolated from porcine tonsils, exhibits distinct characteristics.
Purpose of the Study:
- To investigate the pathogenicity and lesion development of a D. congolensis-like microorganism in mice.
- To compare the morphology of the lesions and microorganisms with D. congolensis.
Main Methods:
- Experimental infection of mice via intraperitoneal and subcutaneous routes.
- Macroscopic and histopathologic examination of induced lesions.
- Microscopic analysis of bacterial morphology within lesions.
Main Results:
- Intraperitoneal and subcutaneous injections resulted in abscess formation.
- Histopathology showed characteristic neutrophil and macrophage arrangements around bacterial colonies.
- Microorganisms exhibited varied morphologies, including mycelial, coccoid, and chlamydospore-like forms.
Conclusions:
- The D. congolensis-like microorganism is pathogenic in mice, inducing abscesses.
- The observed microbial morphology and lesion characteristics differ from typical D. congolensis infections.