Gamma-crystallin-dodecyl sulfate complexes show discrete precipitation in non-ionic detergents
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Updated: Oct 9, 2026

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
Published on: July 4, 2016
Abstract:
Gamma-crystallin-sodium dodecyl sulfate (SDS) complexes are precipitated by Triton X-100 and other non-ionic detergents. This is accomplished as discrete lines by double diffusion, radial diffusion and other immunotechniques. Many other protein-SDS complexes including those of alpha- and beta-crystallins remain soluble. Immunoglobulin G tends to precipitate under similar conditions, a reason why the use of detergents in immunoprecipitation reactions has to be carefully controlled. The phenomenon can be used for the estimation of SDS or non-ionic detergents in the presence of gamma-crystallin.
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