Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutations affecting programmed cell deaths in the nematode Caenorhabditis elegans.

E M Hedgecock, J E Sulston, J N Thomson

    Science (New York, N.Y.)
    |June 17, 1983
    PubMed
    Summary

    Mutations in nematode genes block cell engulfment during development. Programmed cell deaths still occur, indicating cell suicide is the primary mechanism, not phagocytosis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The structure of the nervous system of the nematode Caenorhabditis elegans.

    Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2012
    Same author

    The DNA sequence and biological annotation of human chromosome 1.

    Nature·2006
    Same author

    The fundamental theorem of neutral evolution: rates of substitution and mutation should factor in premeiotic clusters.

    Genetica·2005
    Same author

    DNA sequence and analysis of human chromosome 9.

    Nature·2004
    Same author

    The DNA sequence and analysis of human chromosome 13.

    Nature·2004
    Same author

    The DNA sequence and analysis of human chromosome 6.

    Nature·2003

    Area of Science:

    • Developmental biology
    • Cell biology
    • Genetics

    Background:

    • Programmed cell death (PCD) is crucial for development.
    • Phagocytosis, or cell engulfment, is a key process following PCD.
    • The necessity of phagocytosis for PCD completion is not fully understood.

    Purpose of the Study:

    • To investigate the role of phagocytosis in programmed cell death in nematodes.
    • To identify genetic factors influencing the engulfment of dying cells.

    Main Methods:

    • Utilized genetic screens in nematodes to identify mutations affecting cell engulfment.
    • Observed the fate of cells programmed to die in mutant versus wild-type organisms.

    Main Results:

    • Mutations in two specific nonessential genes completely blocked the phagocytosis of cells undergoing programmed cell death.
    • Despite the lack of engulfment, the affected cells still underwent cell death.
    • These findings were consistent across most observed instances, with few exceptions.

    Conclusions:

    • Cellular engulfment is not essential for the majority of programmed cell deaths in nematodes.
    • Programmed cell deaths in nematodes primarily occur through a mechanism of cell suicide.
    • This suggests a decoupling of cell death execution and subsequent clearance pathways.

    Related Experiment Videos