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Related Experiment Videos

Steroid secretion by mouse testes perfused in vitro.

C Chubb, C Desjardins

    The American Journal of Physiology
    |June 1, 1983
    PubMed
    Summary

    Mouse testes secrete testosterone as the primary androgen, with pregnenolone converted via two pathways. This study comprehensively assesses Leydig cell function and androgen biosynthesis in mice.

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    Area of Science:

    • Endocrinology
    • Reproductive Biology
    • Steroidogenesis

    Background:

    • Leydig cells in the testes are crucial for androgen production.
    • Understanding mouse steroidogenesis provides insights into mammalian reproductive health.

    Purpose of the Study:

    • To determine steroid concentrations in mouse blood plasma.
    • To assess the steroidogenic potential of the mouse testis.
    • To elucidate androgen biosynthesis pathways in mice.

    Main Methods:

    • Perfusing mouse testes with luteinizing hormone and a defined medium.
    • Measuring C18, C19, and C21 steroids in venous effluent.
    • Utilizing chromatography and radioimmunoassay for steroid quantification.

    Main Results:

    • Testosterone is the primary steroid secreted by mouse testes and the main androgen in plasma.
    • Pregnenolone is converted to testosterone through two distinct pathways in roughly equal measure.
    • Identified key steroids in adult mouse circulation, including pregnenolone, androstenedione, and dihydrotestosterone.

    Conclusions:

    • This study offers the first comprehensive assessment of mouse Leydig cell steroidogenic activity.
    • Putative enzymatic pathways for androgen biosynthesis in mice were demonstrated.
    • Established the predominant steroids in adult mouse peripheral circulation.

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