Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue-specific differences in DNA methylation in various mammals.

M A Gama-Sosa, R M Midgett, V A Slagel

    Biochimica Et Biophysica Acta
    |June 24, 1983
    PubMed
    Summary

    Mammalian DNA methylation patterns, specifically 5-methylcytosine, show tissue and species-specific variations. Brain and thymus DNA are hypermethylated, suggesting a role in mammalian differentiation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Parahippocampal gyrus expression of endothelial and insulin receptor signaling pathway genes is modulated by Alzheimer's disease and normalized by treatment with anti-diabetic agents.

    PloS one·2018
    Same author

    Rapid Loading of Molecules into Animal Cells using a Radio-Frequency Electric Field.

    The Biological bulletin·2018
    Same author

    Analysis of gene expression array in TSC2-deficient AML cells reveals IRF7 as a pivotal factor in the Rheb/mTOR pathway.

    Cell death & disease·2014
    Same author

    H-Ras transfers from B to T cells via tunneling nanotubes.

    Cell death & disease·2013
    Same author

    FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease.

    Journal of medical genetics·2010
    Same author

    TYR-MIF-1, but not MIF-1 or morphine, decreases cyclic AMP-dependent protein kinase activity extracted from HeLa cells.

    Molecular and cellular neurosciences·2009

    Area of Science:

    • Epigenetics
    • Molecular Biology
    • Genomics

    Background:

    • Vertebrate DNA contains 5-methylcytosine as its only naturally occurring modified base.
    • DNA methylation is a crucial epigenetic mechanism influencing gene expression and cellular processes.

    Purpose of the Study:

    • To investigate tissue-specific and species-specific differences in DNA methylation in mammals.
    • To explore the relationship between DNA methylation patterns and mammalian differentiation.

    Main Methods:

    • High-performance liquid chromatography (HPLC) was used for precise analysis of DNA.
    • DNA was enzymatically digested into deoxynucleosides for methylation analysis.
    • Methylation levels of cytosine residues were quantified in various tissues of rats, mice, and monkeys.

    Related Experiment Videos

    Main Results:

    • Significant tissue-specific and species-specific variations in DNA methylation were observed across rodents and primates.
    • Brain and thymus DNA exhibited higher methylation levels (hypermethylation) compared to other tissues.
    • Highly repetitive DNA sequences were consistently more methylated than moderately repetitive or single-copy sequences.
    • DNA methylation levels did not correlate with cell turnover rates or transcriptional activity.
    • Liver DNA methylation increased significantly during normal rat development but was unaffected by liver regeneration.

    Conclusions:

    • Tissue-specific DNA methylation patterns are conserved across mammalian species, suggesting a role in differentiation.
    • Hypermethylation in brain and thymus may be a key factor or consequence of mammalian differentiation.
    • Developmental changes significantly impact DNA methylation, potentially due to shifts in cellular composition.