Labelled lipids distribution following [3H]glycerol injection to pregnant rabbits

1. The time dependent variation in specific activity of serum triglycerides and phospholipids has been studied following an intravenous pulse-labelling with 2-[3H]glycerol to pregnant rabbits. 2. The specific activity of triglycerides varied according to the gestation time and the maximal values of specific activity shifted to shorter times when gestation progressed to term. 3. The specific activity of serum phospholipids was higher in pregnant animals and increased with gestation time. 4. Concentrations and specific activities of both triglycerides and phospholipids were investigated in serum of pregnant and fetal rabbits at term. Concentrations of both lipid classes were notably higher in the fetal blood than in the maternal one. 5. The results were discussed on the basis of changes in hepatic biosynthesis of phosphatidic acid. Also, it was suggested a high drainage of either lipids or their degradation products by the feto-placental unit at the end of pregnancy. 6. Levels and specific activities of maternal and fetal lipids from liver and lung were also examined. The different metabolic roles of the main tissues involved in triglyceride and phospholipid metabolism were discussed.