Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 14, 2026

Isolation and Primary Culture of Mouse Aortic Endothelial Cells
08:20

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

Published on: December 19, 2016

Technique for cloning bovine aortic endothelial cells.

C M Gajdusek, S M Schwartz

    In Vitro
    |May 1, 1983
    PubMed
    Summary

    Researchers developed a simple method to isolate pure bovine aortic endothelial cells. This technique ensures high cloning efficiency and significantly boosts cell proliferation in culture.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Big data from small animals: integrating multi-level environmental data into the Dog Aging Project.

    Revue scientifique et technique (International Office of Epizootics)·2023
    Same author

    Muscle-building supplement use and increased risk of testicular germ cell cancer in men from Connecticut and Massachusetts.

    British journal of cancer·2015
    Same author

    Predictive value of bronchoscopy after infant cardiac surgery: a prospective study.

    Intensive care medicine·2012
    Same author

    Pragmatic study of orlistat 60 mg on abdominal obesity.

    European journal of clinical nutrition·2011
    Same author

    Update on the biology and clinical study of restenosis.

    Trends in cardiovascular medicine·2011
    Same author

    Cell proliferation in human atherosclerosis.

    Trends in cardiovascular medicine·2011

    Area of Science:

    • Cell Biology
    • Endothelial Cell Research
    • Tissue Engineering

    Background:

    • Endothelial cells are crucial for vascular health.
    • Establishing pure clonal populations is vital for research.
    • Current methods for endothelial cell isolation can be inefficient.

    Purpose of the Study:

    • To develop a simple and reproducible method for isolating pure clonal bovine aortic endothelial cells.
    • To assess the cloning efficiency of the new method.
    • To evaluate the effect of conditioned medium on endothelial cell proliferation.

    Main Methods:

    • Isolation of single bovine aortic endothelial cells.
    • Culturing cells in conditioned medium.
    • Monitoring cell growth and proliferation.
    • Calculating cloning efficiency and population doubling time.

    Main Results:

    • Achieved virtually 100% cloning efficiency.
    • Demonstrated rapid cell growth with a doubling time of approximately 20 hours.
    • Showed a 40% increase in cell population doubling level using conditioned medium compared to fresh medium.

    Conclusions:

    • The described method provides a highly efficient way to obtain pure clonal bovine aortic endothelial cells.
    • Conditioned medium enhances endothelial cell proliferation, offering a valuable tool for research and potential applications.

    More Related Videos

    Isolation and Culture Expansion of Tumor-specific Endothelial Cells
    10:15

    Isolation and Culture Expansion of Tumor-specific Endothelial Cells

    Published on: October 14, 2015

    Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs
    06:38

    Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs

    Published on: August 14, 2019

    Related Experiment Videos

    Last Updated: Jun 14, 2026

    Isolation and Primary Culture of Mouse Aortic Endothelial Cells
    08:20

    Isolation and Primary Culture of Mouse Aortic Endothelial Cells

    Published on: December 19, 2016

    Isolation and Culture Expansion of Tumor-specific Endothelial Cells
    10:15

    Isolation and Culture Expansion of Tumor-specific Endothelial Cells

    Published on: October 14, 2015

    Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs
    06:38

    Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs

    Published on: August 14, 2019