Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasmids in Frankia sp.

P Normand, P Simonet, J L Butour

    Journal of Bacteriology
    |July 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Two novel pathogenic variants in MED13L: one familial and one isolated case.

    Journal of intellectual disability research : JIDR·2021
    Same author

    Transcriptome of iPSC-derived neuronal cells reveals a module of co-expressed genes consistently associated with autism spectrum disorder.

    Molecular psychiatry·2020
    Same author

    Changing nurse licensing examinations: media analysis and implications of the Canadian experience.

    International nursing review·2017
    Same author

    Genomic imbalances pinpoint potential oncogenes and tumor suppressors in Wilms tumors.

    Molecular cytogenetics·2016
    Same author

    Development and applications of a DNA labeling method with magnetic nanoparticles to study the role of horizontal gene transfer events between bacteria in soil pollutant bioremediation processes.

    Environmental science and pollution research international·2015
    Same author

    Genomic copy number alterations in non-syndromic hearing loss.

    Clinical genetics·2015

    Researchers developed a new method for cell lysis and plasmid DNA isolation in Frankia sp. Four of 39 strains contained plasmids, with 11 plasmids detected in total.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Frankia sp. are nitrogen-fixing bacteria crucial for plant symbiosis.
    • Understanding Frankia sp. plasmids is important for their genetic manipulation and study.
    • Previous methods for plasmid isolation from Frankia sp. may be inefficient.

    Purpose of the Study:

    • To develop and optimize a method for efficient cell lysis and plasmid DNA isolation from Frankia sp.
    • To screen various Frankia sp. strains for the presence of plasmids.
    • To characterize the size and number of detected plasmids.

    Main Methods:

    • Developed a novel cell lysis protocol specifically for Frankia sp.
    • Screened 39 Frankia sp. strains from different host compatibility groups (Alnus sp., Elaeagnus sp., Ceanothus sp.).

    Related Experiment Videos

  • Utilized agarose gel electrophoresis and electron microscopy for plasmid size estimation and detection.

    • Main Results:

    • Successfully developed a method for Frankia sp. cell lysis and plasmid DNA isolation.
    • Identified plasmids in 4 out of 39 screened Frankia sp. strains (Cp11, ARgN22d, ArI3, EUN1f).
    • Detected a total of 11 plasmids, with sizes ranging from 7.1 to 32.2 kilobase pairs.

    Conclusions:

    • The developed method is effective for isolating plasmid DNA from Frankia sp.
    • Plasmids are present in a subset of Frankia sp. strains, varying in size.
    • This work provides a foundation for further research into the function and genetics of Frankia sp. plasmids.